I've had a thought in my head of late. I'm slowly, slowly building my vault up, and I'm sat just under the threshold for Deathclaw attacks. My vault is well equipped room-wise, but I really need to invest in new dwellers now I've got my training rooms set up as my current ones are very weak (didn't have a high Endurance at low enough level). That said I thought it might be a good idea to gun for the one-hundred population mark with a massive population boom to unlock all the rooms so that when the inevitable Deathclaw attacks come and wipe out my vault I've still got access to all the rooms and can rebuild up accordingly.
Just one niggle: I don't know if rooms stay unlocked if you drop back below the unlock threshold. Does anyone have an answer to that?