My favourite quotes from video games are:

Halo 4 - Master Chief: "No, Sir." That I think threw everyone off. Because, y'know, it's the Chief! The Chief don't do [censored] like that!

Skyrim - Ulfric Stormcloak: "Shut up." That line to Elewen was SO BADASS! Obviously everyone is thinking that, but he has the balls to say it.

Movies:

Hellboy 2 -

Abe: "No, no. My body is a temple."

Hellboy: "No, it isn't, it's an amusemant park. Drink up."

13th Warrior:

Ahmed: I cannot lift this.

Herger: Grow stronger!

Also 13th Warrior:

Ahmed: How can you sleep at a time like this?

Herger: The All-Father wove the skein of your life a long time ago. Go and hide in a hole if you wish, but you won't live one instant longer. Your fate is fixed. Fear profits a man nothing.

Video game:

Commander Shepard - Report to the ship as soon as possible. We'll bang, OK?

Cortana - Don't make a girl a promise, if you know you can't keep it.

Movie:

Tropic Thunder - I know who I am! I'm the dude, playing the dude, disguised as another dude.

Iron Man, the Avengers - Are you above or below angry bees?