» Mon Oct 11, 2010 12:05 am
No it will not. This is not how Steam works. You need only connect once to activate/download, then again once every two weeks or so to refresh your login if you choose to use Offline Mode. If you lose login for whatever reason while in online mode Steam will simply retry the connection until it can, your game will go uninterrupted.
Retail copies will install from the disk, however once activated on Steam you may at any time, for any reason, on any computer, download it again. The DVD is useful only as a way to speed up installation should you format your machine. Steam will, as time progresses, download various patches before it allows you to play, once such patches are released. You can, if you want, use the DVD as a coaster while popping the key into Steam and you won't in any way ruin your copy of the game.