There was Only Three?i should read more books in skyrim i thinked that the Nightgale had like 100 members before...Anyway,some very powerfull/skilled dark Brotherhood member could leave the DB and be chooseed by the NightinGale.Don't know,but it's a possibility.Just like a guy from the Thieves Guild,that was once a member of the DB.But left.If i'm not wrong.
Karliah when you join states that the trinity has been restored, indicating that its a group composed of three at any one time, both the stones and platforms in the Nightingale hall all are in 3s, further indicating that its a group composed of three at any time.
Sure a dark brotherhood member could join the thieves guild and become a Nightingale potentially, so could a mage or a warrior though, being an assassin has nothing to do with it, being a great thief however seems to be the prerequisite.