That's how it's supposed to work, but apparently some folks encounter a bug that prevents one or the other ambusher faction from stopping their ambushes. Not that Talon Company will ever be non-hostile towards you, except in that first ambush encounter as a Good character.
I've played a lot of ping pong karma characters that go from evil to good to neutral and back again. I like to hire Jericho and not stay Evil and then do Good stuff, etc. You get the picture. Anyway,what I've seen happen with these characters is that Regulators or Talons will spawn when your karma is at the appropriate level and you're in the cell, but if you don't interact with them they stay there. They don't unspawn or disappear when your karma changes. So if your karma goes back up/down in the meantime you will begin to get spawns of the other group. That means you can have groups of both kinds in your Wasteland at the same time legitimately. I've never been lucky enough to get both kinds at the same spawn point with any of my characters though. I'm not sure how the whole thing is supposed to work I've never bothered to look in the GECK.
Edit: Oh, and I'm not certain it works exactly like this every time. And we've had long discussions about the Talons and Regulators and whether or not they respawn with regularity and we've never been able to figure that out either. They are just odd, by which I mean they are probably buggy which is why some players get the two groups fighting.